Recombinant MERS-CoV S Protein, His-tagged

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Recombinant MERS-CoV S Protein, His-tagged

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Cat.No. : S-016M
Product Overview : Recombinant MERS-CoV Spike S2 Subunit, fused to His-tag at C-terminus, was expressed in insect cell, and purified by using conventional chromatography techniques.
Description : Spike protein S2: mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Source : Insect cells
Species : MERS-CoV
Tag : His
Form : Liquid
Molecular Mass : 60.7kDa
AA Sequence : <ADP>SVPGE MRLASIAFNH PIQVDQLNSS YFKLSIPTNF SFGVTQEYIQ TTIQKVTVDC KQYVCNGFQK CEQLLREYGQ FCSKINQALH GANLRQDDSV RNLFASVKSS QSSPIIPGFG GDFNLTLLEP VSISTGSRSA RSAIEDLLFD KVTIADPGYM QGYDDCMQQG PASARDLICA QYVAGYKVLP PLMDVNMEAA YTSSLLGSIA GVGWTAGLSS FAAIPFAQSI FYRLNGVGIT QQVLSENQKL IANKFNQALG AMQTGFTTTN EAFQKVQDAV NNNAQALSKL ASELSNTFGA ISASIGDIIQ RLDVLEQDAQ IDRLINGRLT TLNAFVAQQL VRSESAALSA QLAKDKVNEC VKAQSKRSGF CGQGTHIVSF VVNAPNGLYF MHVGYYPSNH IEVVSAYGLC DAANPTNCIA PVNGYFIKTN NTRIVDEWSY TGSSFYAPEP ITSLNTKYVA PQVTYQNIST NLPPPLLGNS TGIDFQDELD EFFKNVSTSI PNFGSLTQIN TTLLDLTYEM LSLQQVVKAL NESYIDLKEL GNYTYYNKWP <HHHHHH>
Endotoxin : < 1 EU/μg of protein (determined by LAL method)
Purity : > 85% by SDS-PAGE
Applications : SDS-PAGE
Storage : Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -95 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.25 mg/mL (determined by Absorbance at 280nm)
Storage Buffer : Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Official Symbol : S
Synonyms : Middle East respiratory syndrome coronavirus; Human betacoronavirus 2c EMC/2012; MERS-CoV; MERS; MERSCoV S2 P; Spike2 glycoprotein; S2 glycoprotein; S2; Spike S2 Subunit protein; S2 Subunit
UniProt ID : K0BRG7
SDS-PAGE :

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain

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