Recombinant MERS-CoV Spike/S1 protein(Met1-Glu725), His-tagged

• Cat.No.: Spike-1228V
• Product Overview: Recombinant spike protein S1 Subunit MERS-CoV (AFS88936.1)(Met1-Glu725) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: MERS-CoV
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Glu725
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) at 10 μg/ml (100 μl/well) can bind biotinylated human DPP4. The EC50 of of biotinylated DPP4 is 0.52-1.22 μg/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) at 10 μg/ml (100 μl/well) can bind biotinylated Fc-DPP4. The EC50 of biotinylated Fc-DPP4 is 0.02-0.06 μg/ml.
• Molecular Mass: The recombinant spike protein S1 Subunit MERS-CoV comprises 719 amino acids and has a predicted molecular mass of 79.9 kDa. It migrates as an approximately 94 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.