Recombinant Microbacterium Testaceum AIIM Protein (1-251 aa), His-SUMO-tagged

• Cat.No.: AIIM-1776M
• Product Overview: Recombinant Microbacterium Testaceum (strain StLB037) AIIM Protein (1-251 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Full Length.

Specification

• Description: Hydrolyzes short- and long-chain N-acyl homoserine lactones with or without 3-oxo substitution at C3. Has slight activity towards L-homoserine lactone, and no activity towards gamma-butyrolactone.
• Source: E. coli
• Species: Microbacterium Testaceum
• Tag: His-SUMO
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 43.3 kDa
• Protein length: 1-251 aa
• AA Sequence: MILAHDVSGSGPLLVLLHGITEDRR SWDPVDFTDGFTVVRVDLRGHGASA AEEPYDIPTLATDVHDTLAQLAEND VIPGELPVIVGHSMGGIVATAYGAL FPARAIVNVDQPLQLAGMQGQVQQA EGMLRGADFPLFIHGMFAQMAGGLD AEELARVNGIRSPRQDVVLGMWRPL LEDSPEELAALVSGLTRIPEDVPYL VITGLDAGPEYAAWLQREIPQAVQE VWQPPTHYPHLVDPARFVERVEAFV R
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.
• Synonyms: aiiM; AHL-lactonase
• UniProt ID: C6L862

Gene Information

• Synonyms: aiiM; AHL-lactonase
• UniProt ID: C6L862

Reviews

04/22/2022

By incorporating the high-quality AIIM protein alongside the manufacturer's outstanding technical support, I am confident in surpassing potential obstacles and achieving my research objectives effortlessly.

10/21/2020

This protein serves as a crucial asset in unraveling the intricate mechanisms associated with aiiA, augmenting the existing knowledge in this field.

09/08/2019

Their prompt and effective assistance lends itself to a seamless workflow, ensuring efficient troubleshooting and data analysis.

Q&As

Are there any regulatory considerations for the use of AIIM protein in algal bloom control?

As AIIM protein is a new technology, there may be regulatory considerations for its use in algal bloom control. Before use in aquatic environments, regulatory approval may be required to ensure that the protein does not have negative impacts on human health or the environment.

Can AIIM protein be used in combination with other algal bloom control methods?

Yes, AIIM protein may be used in combination with other algal bloom control methods, such as physical removal of algae or water treatment methods. Combining methods may enhance effectiveness and reduce negative impacts on non-target organisms.

Can AIIM protein be used in combination with other treatments for algal bloom control?

Yes, AIIM protein can be used in combination with other treatments for algal bloom control, such as nutrient limitation and physical removal methods.

Are there any known negative impacts of AIIM protein on non-target species in aquatic environments?

While some studies have suggested that AIIM protein may have selectivity towards certain algal species and not harm non-target species, more research is needed to fully understand the potential impacts of AIIM protein on non-target organisms in aquatic environments.

How does AIIM protein work to control algal blooms?

AIIM protein is a biological control method that uses a specific protein, called AiiM, to target and disrupt the signaling pathways of harmful algal blooms. The protein binds to a specific molecule called autoinducer, which is released by algae to communicate and coordinate their actions.

How is AIIM protein produced?

AIIM protein is produced by genetically modifying bacteria to express the AiiM protein. The bacteria then produce and secrete the protein, which can be extracted and purified for use in algal bloom control.

What are some challenges to the production and application of AIIM protein?

Some challenges to the production and application of AIIM protein include the cost of production, variability in the effectiveness of the protein on different algal species, potential negative impacts on non-target organisms, and the need for further research to fully understand the protein's potential role in algal bloom control.

How does AIIM protein compare to other algal bloom control methods?

AIIM protein has some advantages over other algal bloom control methods, such as its potential selectivity to certain algal species and its ability to target harmful algal blooms without impacting non-target organisms. However, it also has limitations, such as the need for large quantities of protein and the potential development of algal resistance over time. Each method has its own advantages and disadvantages, and the most effective approach may depend on the specific situation.

What are the potential applications of AIIM protein?

AIIM protein has potential applications in algal bloom control, aquaculture, and bioremediation. It can be used to control harmful algal blooms in aquatic environments, to prevent the growth of unwanted algae in aquaculture operations, and to remove excess algae in polluted waters.

Are there any known risks associated with the use of AIIM protein in algal bloom control?

While AIIM protein has shown promise as an algal bloom control agent, more research is needed to fully evaluate the potential risks associated with its use. Some risks could include negative impacts on non-target organisms, the development of algal resistance, and the need for large quantities of protein.

Are there any potential limitations to the use of AIIM protein in algal bloom control?

Yes, there are potential limitations to the use of AIIM protein in algal bloom control, including the need for a large quantity of protein, the cost of production, and the potential for the development of resistant algal strains over time.

What are the environmental conditions required for optimal production of AIIM protein?

The optimal environmental conditions for AIIM protein production may vary depending on the specific bacterial strain. However, some studies have suggested that AIIM protein production is enhanced by certain metal ions in the environment, such as iron and copper.

How is AIIM protein produced?

AIIM protein is produced by marine bacteria, and can be extracted and purified from bacterial cultures.

Has AIIM protein been tested in field trials?

AIIM protein has been tested in laboratory studies and small-scale field trials, but larger-scale trials are needed to fully evaluate its effectiveness and potential risks.

Are there any ethical concerns related to the use of AIIM protein in algal bloom control?

As with any new technology, there may be ethical considerations related to the use of AIIM protein in algal bloom control. Some concerns may include the potential impact on non-target organisms, the possible development of algal resistance, and the need for thorough testing and evaluation before widespread use. It is important to consider these concerns and any potential risks before implementing the use of AIIM protein in algal bloom control.