Recombinant Monkey ERBB2 Protein, Fc-tagged, Alexa Fluor 488 conjugated

• Cat.No.: ERBB2-1216CAF488
• Product Overview: Recombinant Monkey ERBB2 Protein (1-652aa, Rhesus ERBB2 XP_001090430.1 with Asp121Asn/Leu122Pro mutation, similar to Macaca fascicularis ERBB2 EHH58073.1), was expressed in HEK293 with C-terminal human IgG1 Fc tag and Alexa Fluor 488 conjugate.​

Specification

• Species: Monkey
• Source: HEK293
• Tag: Fc
• Protein Length: 1-652 aa
• Form: Lyophilized
• Molecular Mass: 96.4 kDa
• N-terminal Sequence Analysis: Thr 23
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Alexa Fluor 488

Gene Information

• Gene Name: ERBB2 erb-b2 receptor tyrosine kinase 2 [ Macaca fascicularis ]
• Official Symbol: ERBB2
• Synonyms: erb-b2 receptor tyrosine kinase 2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
• Gene ID: 102146608

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.