Recombinant Mouse ADP-ribosyltransferase 2b, His-tagged

• Cat.No.: Art2b-2068M
• Product Overview: Recombinant MouseART2.2 is produced inE. coliwith aC-terminus His-tag.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: Extracellular NAD induces the ATP-independent activation of the ionotropic P2X(7) purinergic receptor (P2X(7)R) in murine T lymphocytes via a novel covalent pathway involving ADP-ribosylation of arginine residues on the P2X(7)R ectodomain. This modification is catalyzed by ART2.2, a GPI-anchored ADP-ribosyltransferase (ART) that is constitutively expressed in murine T cells. We previously reported that ART2.1, a related ecto-ART, is up-regulated in inflammatory murine macrophages that constitutively express P2X(7)R. Thus, we tested the hypothesis that extracellular NAD acts via ART2.1 to regulate P2X(7)R function in murine macrophages. Coexpression of the cloned murine P2X(7)R with ART2.1 or ART2.2 in HEK293 cells verified that P2X(7)R is an equivalent substrate for ADP-ribosylation by either ART2.1 or ART2.2. However, in contrast with T cells, the stimulation of macrophages or HEK293 cells with NAD alone did not activate the P2X(7)R. Rather, NAD potentiated ATP-dependent P2X(7)R activation as indicated by a left shift in the ATP dose-response relationship.
• Concentration: 0.5mg/ml.
• Formulation: Liquid. In PBS containing 50% glycerol.
• Purity: ≥98%.
• Storage: -80°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: Art2b ADP-ribosyltransferase 2b [ Mus musculus ]
• Synonyms: Art2b; ADP-ribosyltransferase 2b; T-cell NAD(P)(+)--arginine ADP-ribosyltransferase 2; T-cell differentiation marker Rt6 homolog 2; T-cell ecto-ADP-ribosyltransferase 2; T-cell mono(ADP-ribosyl)transferase 2; mono(ADP-ribosyl)transferase 2B; Art; Rt6; Rt-6; Ly92b; Rt6-2; ART2.2; Art2b
• Gene ID: 11872
• mRNA Refseq: NM_019915
• Protein Refseq: NP_064299
• UniProt ID: O35975
• Chromosome Location: 7 E3; 7 49.0 cM
• Function: NAD(P)+-protein-arginine ADP-ribosyltransferase activity; transferase activity; transferase activity, transferring glycosyl groups