Recombinant Mouse ALYREF Protein

• Cat.No.: ALYREF-1584M
• Product Overview: Recombinant Mouse ALYREF full length or partial length protein was expressed.

Specification

• Source: Mammalian Cells
• Species: Mouse
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 ºC for short term. For long term storage, store it at -20 ºC～-80 ºC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: Alyref Aly/REF export factor [ Mus musculus ]
• Official Symbol: ALYREF
• Gene ID: 21681
• mRNA Refseq: NM_011568.1
• Protein Refseq: NP_035698.1
• UniProt ID: O08583

Reviews

10/02/2022

Enhanced signal amplification for improved sensitivity.

01/04/2022

Precise determination of protein half-life.

09/14/2018

Compatibility with various downstream applications (e.g., mass spectrometry).

Q&As

What are the interacting partners of ALYREF, and how do these interactions regulate its function in mRNA export or other cellular processes?

ALYREF interacts with other mRNA export factors, such as NXF1, NXT1, and UAP56, as well as RNA binding proteins, transcription factors, and RNA helicases to coordinate mRNA export processes.

How does ALYREF contribute to the regulation of gene expression, and what are the target genes or pathways affected by its function?

ALYREF contributes to the regulation of gene expression by influencing the export efficiency of specific mRNA subsets, thereby impacting their translation and protein production.

Can ALYREF be targeted pharmacologically to modulate mRNA export and potentially influence gene expression patterns in disease contexts?

Targeting ALYREF pharmacologically may offer a means to modulate mRNA export and gene expression, potentially influencing disease-related processes, including cancer progression or viral replication.

What are the cellular signaling pathways or cues that regulate the expression, localization, or activity of ALYREF in response to specific cellular stimuli?

ALYREF expression, localization, or activity can be regulated by signaling pathways, such as the PI3K/AKT/mTOR pathway or stress response pathways, to modulate mRNA export under specific conditions.

What are the post-translational modifications of ALYREF, and how do they impact its localization, stability, or RNA binding affinity?

ALYREF undergoes phosphorylation, acetylation, and methylation, which can regulate its RNA binding affinity, nuclear-cytoplasmic shuttling, or interactions with other proteins.

What are the consequences of ALYREF depletion or overexpression on cellular processes such as proliferation, differentiation, or response to stress?

ALYREF depletion or overexpression can lead to defects in mRNA export, altered gene expression patterns, and impaired cellular processes, such as compromised proliferation, impaired differentiation, or increased sensitivity to stress.

What are the disease-relevant implications of ALYREF dysfunction, and can it serve as a diagnostic or prognostic marker for certain conditions?

ALYREF dysfunction has been implicated in various diseases, including cancer, neurological disorders, and viral infections. Its altered expression or subcellular localization may serve as diagnostic or prognostic markers in certain disease contexts.

How does ALYREF contribute to mRNA export from the nucleus to the cytoplasm, and what are the key molecular players involved in this process?

ALYREF plays a crucial role in mRNA export by interacting with mRNA export factors, nuclear pore components, and RNA molecules to facilitate their transport across the nuclear envelope.

How does ALYREF participate in the quality control of mRNA export, and what are the consequences of its dysfunction on cellular mRNA metabolism?

ALYREF participates in mRNA surveillance mechanisms, including the recognition and removal of aberrant or misprocessed mRNAs, preventing their export to the cytoplasm.

What are the specific RNA binding motifs or domains within ALYREF, and how do they interact with mRNA molecules to facilitate their export?

ALYREF contains RNA recognition motifs (RRMs) that bind to specific RNA sequences or structures, such as exon junction complexes (EJCs) or specific mRNA export elements.