Recombinant Mouse App Protein, His Tagged

• Cat.No.: App-11208M
• Product Overview: Mouse N-APP, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 18-Val 286 (Accession # P12023-1). This protein carries a polyhistidine tag at the C-terminus. Predicted N-terminus: Leu 18

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 18-286 aa
• Description: Amyloid precursor protein (APP) is a type I integral membrane protein ubiquitously expressed in many tissues and concentrated in the synapses of neurons. The majority of APP is cleaved at the plasma membrane by the α-secretase in the non-amyloidogenic pathway. The amyloidogenic pathway starts with β-secretase cleavage by BACE1 on the N-terminal part of the Aβ domain, releasing sAPPβ from a membrane-anchored fragment named βCTF or C102, which is subsequently cleaved by γ-secretase to release Aβ.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Molecular Mass: The protein has a calculated MW of 31.9 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >95% as determined by SDS-PAGE. >95% as determined by SEC-MALS.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: App amyloid beta (A4) precursor protein [ Mus musculus (house mouse) ]
• Official Symbol: App
• Synonyms: APP; amyloid beta (A4) precursor protein; amyloid beta A4 protein; appican; protease nexin II; amyloidogenic glycoprotein; alzheimer disease amyloid A4 protein homolog; Ag; Abpp; Adap; Cvap; Abeta; betaApp; E030013M08Rik
• Gene ID: 11820
• mRNA Refseq: NM_001198823
• Protein Refseq: NP_001185752
• UniProt ID: P12023