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Recombinant Mouse B4galt2 overexpression lysate

Cat.No. : B4galt2-11HCL
Product Overview : Recombinant mouse B4galt2 HEK293T cell transient overexpression lysate (as WB positive control) with a C-Myc/DDK tag was expressed in HEK293T.
Preparation: HEK293T cells in 10-cm dishes were transiently transfected. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Components: 1 vial of 20 μg lyophilized gene specific transient over-expression cell lysate
  • Specification
  • Gene Information
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Description : Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose.
Source : HEK293T
Species : Mouse
Tag : Myc/DDK
Molecular Mass : 42.4 kDa
Stability : Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles after reconstitution.
Storage : Upon receiving, store the sample at -20 centigrade.
Reconstitution : Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Shipping : The lysate can be shipped at ambient temperature.
Gene Name : B4galt2 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2 [ Mus musculus (house mouse) ]
Official Symbol : B4galt2
Synonyms : B4galt2; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 2; Ggtb2; beta-1,4-galactosyltransferase 2; N-acetyllactosamine synthase; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2; b4Gal-T2; beta-1,4-GalTase 2; beta-1,4-galactosyltransferase II; beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase; beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase; beta4Gal-T2; lactose synthase A protein; nal synthase; EC 2.4.1.22; EC 2.4.1.38; EC 2.4.1.90
Gene ID : 53418
mRNA Refseq : NM_017377
Protein Refseq : NP_059073
UniProt ID : Q9Z2Y2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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