Recombinant Mouse B4galt3 overexpression lysate

• Cat.No.: B4galt3-22HCL
• Product Overview: Transient overexpression lysate of Mouse UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3 (B4galt3) with a C-Myc/DDK tag was expressed in HEK293T.
  Preparation: HEK293T cells in 10-cm dishes were transiently transfected. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20 centigrade before shipping.
  Components:
  1 vial of 100 μg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 μg empty vector transfected control cell lysate in RIPA buffer
  1 vial of 250 μL 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)

Specification

• Description: Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 44.1 kDa
• Stability: Avoid repeated freeze-thaw cycles. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade.
• Storage: Upon receiving, store the sample at -20 centigrade.
• Reconstitution: Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage.
• Shipping: The lysate is shipped with dry ice.

Gene Information

• Gene Name: B4galt3 UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3 [ Mus musculus (house mouse) ]
• Official Symbol: B4galt3
• Synonyms: B4galt3; UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3; ESTM2; ESTM6; R7498; ESTM26; R74981; AA104562; AW125175; 9530061M23Rik; beta-1,4-galactosyltransferase 3; N-acetyllactosamine synthase; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3; UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 3; beta-1,4-galactosyltransferase III; beta4GalT-III; expressed sequence tag mouse EST 6; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3; b4Gal-T3; beta-1,4-GalTase 3; beta-1,4-galactosyltransferase III; beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase; beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase; beta4Gal-T3; beta4GalT-III; nal synthase; neolactotriaosylceramide beta-1,4-galactosyltransferase; EC 2.4.1.275; EC 2.4.1.38; EC 2.4.1.90
• Gene ID: 57370
• mRNA Refseq: NM_020579
• Protein Refseq: NP_065604
• UniProt ID: Q91YY2