Recombinant Mouse B4galt7 overexpression lysate

• Cat.No.: B4galt7-19HCL
• Product Overview: Transient overexpression lysate of Mouse xylosylprotein beta1,4-galactosyltransferase, polypeptide 7 (galactosyltransferase I) (B4galt7) with a C-Myc/DDK tag was expressed in HEK293T.
  Preparation: HEK293T cells in 10-cm dishes were transiently transfected. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20 centigrade before shipping.
  Components:
  1 vial of 100 μg gene specific transient over-expression cell lysate in RIPA buffer
  1 vial of 100 μg empty vector transfected control cell lysate in RIPA buffer
  1 vial of 250 μL 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)

Specification

• Description: Required for the biosynthesis of the tetrasaccharide linkage region of proteoglycans, especially for small proteoglycans in skin fibroblasts.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 37.8 kDa
• Stability: Avoid repeated freeze-thaw cycles. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade.
• Storage: Upon receiving, store the sample at -20 centigrade.
• Reconstitution: Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage.
• Shipping: The lysate is shipped with dry ice.

Gene Information

• Gene Name: B4galt7 xylosylprotein beta1,4-galactosyltransferase, polypeptide 7 (galactosyltransferase I) [ Mus musculus (house mouse) ]
• Official Symbol: B4galt7
• Synonyms: B4galt7; xylosylprotein beta1,4-galactosyltransferase, polypeptide 7 (galactosyltransferase I); XGPT; XGalT-1; beta-1,4-galactosyltransferase 7; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 7; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 7; UDP-galactose:beta-xylose beta-1,4-galactosyltransferase; b4Gal-T7; beta-1,4-GalTase 7; beta4Gal-T7; proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I; xylosylprotein 4-beta-galactosyltransferase; xylosylprotein beta-1,4-galactosyltransferase; EC 2.4.1.133
• Gene ID: 218271
• mRNA Refseq: NM_146045
• Protein Refseq: NP_666157
• UniProt ID: Q8R087