Recombinant Mouse Bmt2 Protein, MYC/DDK-tagged

• Cat.No.: Bmt2-173M
• Product Overview: Purified recombinant protein of full-length mouse base methyltransferase of 25S rRNA 2 (Bmt2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: S-adenosyl-L-methionine-binding protein that acts as an inhibitor of mTORC1 signaling via interaction with the GATOR1 and KICSTOR complexes. Acts as a sensor of S-adenosyl-L-methionine to signal methionine sufficiency to mTORC1: in presence of methionine, binds S-adenosyl-L-methionine, leading to disrupt interaction with the GATOR1 and KICSTOR complexes and promote mTORC1 signaling. Upon methionine starvation, S-adenosyl-L-methionine levels are reduced, thereby promoting the association with GATOR1 and KICSTOR, leading to inhibit mTORC1 signaling. Probably also acts as a S-adenosyl-L-methionine-dependent methyltransferase.
• Molecular Mass: 46 kDa
• Purity: >80%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Bmt2 base methyltransferase of 25S rRNA 2 [ Mus musculus (house mouse) ]
• Official Symbol: Bmt2
• Synonyms: Bmt2; base methyltransferase of 25S rRNA 2; AI666701; B630005N14Rik; S-adenosylmethionine sensor upstream of mTORC1; UPF0532 protein C7orf60 homolog; probable methyltransferase BMT2 homolog; probable methyltransferase BTM2 homolog; EC 2.1.1.-
• Gene ID: 101148
• mRNA Refseq: NM_175312
• Protein Refseq: NP_780521
• UniProt ID: Q8BXK4