Recombinant Mouse Car4 Protein, MYC/DDK-tagged

• Cat.No.: Car4-767M
• Product Overview: Purified recombinant protein of full-length mouse carbonic anhydrase 4 (Car4), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Biased expression in colon adult (RPKM 148.4), placenta adult (RPKM 86.4) and 10 other tissues.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 34.4 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Car4 carbonic anhydrase 4 [ Mus musculus (house mouse) ]
• Official Symbol: Car4
• Synonyms: Car4; carbonic anhydrase 4; Ca4; AW456718; carbonic anhydrase 4; CA-IV; carbonate dehydratase IV; carbonic anhydrase IV; EC 4.2.1.1
• Gene ID: 12351
• mRNA Refseq: NM_007607
• Protein Refseq: NP_031633
• UniProt ID: Q64444

Reviews

04/14/2022

Reliable partner for protein expression and analysis.

04/12/2022

Cost-effective quantitation services, a great research investment.

12/09/2020

Invaluable for our protein analysis and quantification endeavors.

Q&As

What role does CA4 play in the respiratory system?

CA4 assists in CO2 and bicarbonate regulation, pivotal for efficient respiratory gas exchange.

Which tissues or cell types predominantly express CA4?

CA4 is highly expressed in the kidney and red blood cells.

What is the primary physiological function of CA4?

CA4 catalyzes the reversible hydration of carbon dioxide, helping regulate pH and CO2 transport.

What are the known post-translational modifications on CA4?

Typical modifications like phosphorylation can be identified using techniques such as mass spectrometry.

Is CA4 implicated in any specific cellular signaling pathways?

While primarily enzymatic, any signaling roles for CA4 would require deeper investigation.

Are there known diseases or conditions associated with CA4 mutations or dysregulation?

CA4 dysregulation could relate to renal disorders or hematological abnormalities, though research is ongoing.

How does the enzymatic activity of CA4 compare to other carbonic anhydrase family members?

CA4, like other isoforms, is involved in CO2 hydration, but its specific efficiency and affinity might vary.