Recombinant Mouse Car9 Protein, His-tagged, FITC conjugated

Cat.No. : Car9-914MF
Product Overview : FITC conjugated recombinant Mouse Car9 (NP_647466.2) extracellular domain (Met 1-Asp 390), fused with a C-terminal polyhistidine tag, was produced in Human Cell.
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Source : HEK293
Species : Mouse
Tag : His
Form : Lyophilized
Molecular Mass : The secreted recombinant mouse CAR9 comprises 370 amino acids and has a calculated molecular mass of 40.3 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 45-50 kDa in SDS-PAGE under reducing conditions.
Protein length : 370
Endotoxin : < 1.0 EU/ μg of the protein as determined by the LAL method.
Characteristic : Disulfide-linked homodimer
Labeled with FITC via amines
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Stability : Samples are stable for up to 12 months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Storage Buffer : Lyophilized from sterile PBS, pH 7.4
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name : Car9 carbonic anhydrase 9 [ Mus musculus ]
Official Symbol : Car9
Gene ID : 230099
mRNA Refseq : NM_139305
Protein Refseq : NP_647466

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What signaling pathways are known to regulate CA9 expression? 06/15/2022

The HIF-1 signaling pathway primarily regulates CA9 in response to cellular oxygen levels.

How does CA9 contribute to pH regulation within the tumor microenvironment? 02/02/2022

CA9 aids in extracellular acidification, promoting tumor cell survival and invasion in acidic tumor microenvironments.

How is CA9 expression regulated in hypoxic conditions? 04/15/2021

CA9 expression is upregulated by the hypoxia-inducible factor (HIF) under low oxygen conditions.

How are elevated levels of CA9 associated with cancer prognosis? 05/26/2020

Elevated CA9 levels often correlate with poor prognosis in various cancers due to its role in tumor adaptation to hypoxia.

What post-translational modifications have been identified on CA9? 11/23/2017

Potential modifications like glycosylation can be identified using techniques like mass spectrometry.

Are there specific inhibitors that target CA9, especially in cancer therapeutics? 09/29/2017

Several inhibitors, like acetazolamide derivatives, have been explored to target CA9 in cancer treatment.

What roles does CA9 play in tumor physiology? 07/06/2017

CA9 helps tumors survive in hypoxic conditions by regulating pH through the conversion of CO2 to bicarbonate and protons.

Customer Reviews (3)

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08/04/2022

    Exceptional consistency, vital for our research's reliability.

    12/16/2021

      Critical support for our protein analysis and quantification.

      02/07/2021

        Unmatched accuracy in protein identification and sequencing.

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