Recombinant Mouse Ccl9 protein

• Cat.No.: Ccl9-619M
• Product Overview: Recombinant Mouse Ccl9 protein was expressed in Escherichia coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Protein Length: 101
• Description: Murine CCL9/10 is belonging to the CC chemokine family. It is encoded by the gene CCL9. MRP-2 (CCL9/10) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 shows a 51 % sequence identity at the protein level to MRP-1 (C10) and 46% identity to MIP-1-alpha (CCL3). The functional receptor for CCL9/10 has been identified as CCR1. In contrast, other CCR1 ligands, CCL3/MIP-1alpha and CCL5/RANTES are considered to be primary CCR1 ligands in inflammatory responses. CCL9 can activate osteoclasts suggesting an important role for it in bone resorption. Murine CCL9/10 is 75 % amino acid identical to rat CCL9/10.
• Form: Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
• Bio-activity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml.
• Molecular Mass: Approximately 11.6 kDa, a single non-glycosylated polypeptide chain containing 101 amino acids.
• AA Sequence: QITHATETKEVQSSLKAQQGLEIEMFHMGFQDSSDCCLSYNSRIQCSRFIGYFPTSGGCTRPGIIFISKRGFQVCANPSDRRVQRCIERLEQNSQPRTYKQ
• Endotoxin: Less than 1 EU/μg of rMuMIP-1γ/CCL9/CCL10 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: Ccl9
• Official Symbol: Ccl9
• Synonyms: CCL9; chemokine (C-C motif) ligand 9; C-C motif chemokine 9; MIP-1 gamma; small inducible cytokine A9; small inducible cytokine A10; macrophage inflammatory protein 1-gamma; macrophage inflammatory protein-related protein 2; CCF18; MRP-2; Scya9; Scya10
• Gene ID: 20308
• mRNA Refseq: NM_011338
• Protein Refseq: NP_035468
• UniProt ID: P51670