Recombinant Mouse Ccl9 protein

Cat.No. : Ccl9-619M
Product Overview : Recombinant Mouse Ccl9 protein was expressed in Escherichia coli.
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Description : Murine CCL9/10 is belonging to the CC chemokine family. It is encoded by the gene CCL9. MRP-2 (CCL9/10) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 shows a 51 % sequence identity at the protein level to MRP-1 (C10) and 46% identity to MIP-1-alpha (CCL3). The functional receptor for CCL9/10 has been identified as CCR1. In contrast, other CCR1 ligands, CCL3/MIP-1alpha and CCL5/RANTES are considered to be primary CCR1 ligands in inflammatory responses. CCL9 can activate osteoclasts suggesting an important role for it in bone resorption. Murine CCL9/10 is 75 % amino acid identical to rat CCL9/10.
Source : E.coli
Species : Mouse
Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Bio-activity : Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml.
Molecular Mass : Approximately 11.6 kDa, a single non-glycosylated polypeptide chain containing 101 amino acids.
Protein length : 101
AA Sequence : QITHATETKEVQSSLKAQQGLEIEM FHMGFQDSSDCCLSYNSRIQCSRFI GYFPTSGGCTRPGIIFISKRGFQVC ANPSDRRVQRCIERLEQNSQPRTYK Q
Endotoxin : Less than 1 EU/μg of rMuMIP-1γ/CCL9/CCL10 as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
SDS-PAGE:
Gene Name : Ccl9
Official Symbol : Ccl9
Synonyms : CCL9; chemokine (C-C motif) ligand 9; C-C motif chemokine 9; MIP-1 gamma; small inducible cytokine A9; small inducible cytokine A10; macrophage inflammatory protein 1-gamma; macrophage inflammatory protein-related protein 2; CCF18; MRP-2; Scya9; Scya10;
Gene ID : 20308
mRNA Refseq : NM_011338
Protein Refseq : NP_035468
UniProt ID : P51670

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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