Active Recombinant Mouse Cd27 protein, mFc-tagged, Low Endotoxin

• Cat.No.: Cd27-5654M
• Product Overview: Recombinant Mouse Cd27 protein(P41272-1)(24-182 aa), fused with C-terminal mFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: mFc
• Protein Length: 24-182 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse CD27, mFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 34.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Cd27 CD27 antigen [ Mus musculus ]
• Official Symbol: Cd27
• Synonyms: CD27; CD27 antigen; CD antigen 27; CD27L receptor; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7; S152; Tp55; Tnfrsf7
• Gene ID: 21940
• mRNA Refseq: NM_001033126
• Protein Refseq: NP_001028298