Active Recombinant Mouse Cd274 protein, mFc-tagged

• Cat.No.: Cd274-359M
• Product Overview: Recombinant Mouse Cd274 protein(Phe 19 - Thr 238), fused with mFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: mFc
• Protein Length: Phe 19 - Thr 238
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Rat PD-1, His Tag at 2 μg/mL (100 μL/well) can bind Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 20-625 ng/mL. Immobilized Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse PD-1, Fc,Avitag with a linear range of 0.313-5 μg/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminusThe protein has a calculated MW of 51.8 kDa. The protein migrates as 65-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd274 CD274 antigen [ Mus musculus ]
• Official Symbol: Cd274
• Synonyms: CD274; CD274 antigen; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• Gene ID: 60533
• mRNA Refseq: NM_021893
• Protein Refseq: NP_068693