Recombinant Mouse Cd276 Protein, hFc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: CD276-1287MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse CD276 (Val29-Phe244) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. It could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy.
• Source: HEK293
• Species: Mouse
• Tag: hFc
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 50.1 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Val29-Phe244
• N-terminal Sequence Analysis: Val 29
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Cd276 CD276 antigen [ Mus musculus (house mouse) ]
• Official Symbol: Cd276
• Synonyms: CD276; CD276 antigen; B7-H3; B7 homolog 3; costimulatory molecule; B7h3; B7RP-2; AU016588; 6030411F23Rik
• Gene ID: 102657
• mRNA Refseq: NM_133983
• Protein Refseq: NP_598744
• UniProt ID: Q8VE98