Recombinant Mouse Cd28 Protein, His-tagged, Alexa Fluor 488 conjugated

• Cat.No.: Cd28-4008MAF488
• Product Overview: Alexa Fluor 488 conjugated recombinant Mouse Cd28 (Asn20-Lys149) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 16.9 kDa. The protein migrates as 30-35 kDa and 55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Asn20-Lys149
• N-terminal Sequence Analysis: Asn 20
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515­-545 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Cd28
• Official Symbol: Cd28
• Synonyms: CD28; CD28 antigen; T-cell-specific surface glycoprotein CD28
• Gene ID: 12487
• mRNA Refseq: NM_007642
• Protein Refseq: NP_031668
• UniProt ID: P31041

Reviews

08/24/2021

Its unique characteristics contribute to obtaining clear and detailed structural information, enabling researchers to unravel the intricate complexities of protein interactions and conformational changes.

08/24/2021

Researchers can confidently rely on its capabilities to generate precise and significant results, enhancing the understanding of protein function and dynamics.

08/06/2021

the CD28 protein's outstanding performance in ELISA and its utility in protein electron microscopy structure analysis make it a highly recommended tool for researchers.

Q&As

Can CD28 protein be used for personalized medicine?

CD28 protein expression and function may be relevant for personalized treatment strategies in autoimmune diseases and cancer.

What are the potential clinical applications of targeting CD28 protein?

Targeting CD28 protein may have therapeutic potential in treating autoimmune diseases, cancer immunotherapy, and organ transplantation.

How does CD28 protein impact regulatory T cells (Tregs)?

CD28 signaling is important for the function and maintenance of regulatory T cells, which play a role in immune tolerance and suppression of autoimmunity.

How does CD28 protein impact cancer immunotherapy?

CD28 protein is involved in enhancing the anti-tumor immune response, making it a potential target for cancer immunotherapy.

Are there any approved therapies targeting CD28 protein?

Currently, there are no FDA-approved therapies specifically targeting CD28 protein, but research is ongoing in this area.