Recombinant Mouse Cdk8 Protein, Myc/DDK-tagged

• Cat.No.: Cdk8-2091M
• Product Overview: Purified recombinant protein of mouse full-length cyclin-dependent kinase 8 (Cdk8), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 53.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cdk8 cyclin-dependent kinase 8 [ Mus musculus (house mouse) ]
• Official Symbol: Cdk8
• Synonyms: CDK8; cyclin-dependent kinase 8; mediator complex subunit CDK8; cell division protein kinase 8; mediator of RNA polymerase II transcription subunit CDK8; MGC37111
• Gene ID: 264064
• mRNA Refseq: NM_153599
• Protein Refseq: NP_705827
• UniProt ID: Q8R3L8