|Product Overview:||Recombinant Mouse Cfl1(non-muscle isoform) fused with GST tag at the N-terminus was produced in E. coli.|
|Description:||Cofilin depolymerizes actin filaments and inhibits polymerization of G-actin in a pH-sensitive manner. It is a regulatory protein of cytoskeletal actin dynamics. Cofilin plays a role in the regulation of cell morphology and cytoskeletal organization.|
|Form:||150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 1 ml ultrapure water to obtain a 1.0 mg/ml solution.|
|Purity:||Purity: >96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
Purification notes: Cofilin was expressed as a GST-tag fusion protein in E. coli. After purification by GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography.
|Storage:||Cofilin is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20 centigrade in glycerol or CryoProtect. Avoid repeated freeze / thaw cycles.|
|Shipping:||At ambient temperature. Upon delivery store at -70 centigrade.|
|Gene Name:||Cfl1 cofilin 1, non-muscle [ Mus musculus ]|
|Synonyms:||CFL1; cofilin 1, non-muscle; cofilin-1; cofilin, non-muscle isoform; Cof; AA959946;|
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