||Recombinant Mouse Cfl1(non-muscle isoform) fused with GST tag at the N-terminus was produced in E. coli.
||Cofilin depolymerizes actin filaments and inhibits polymerization of G-actin in a pH-sensitive manner. It is a regulatory protein of cytoskeletal actin dynamics. Cofilin plays a role in the regulation of cell morphology and cytoskeletal organization.
||150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 1 ml ultrapure water to obtain a 1.0 mg/ml solution.
||Purity: >96% by scanning densitometry from Coomassie G-250 stained SDS-Gels.
Purification notes: Cofilin was expressed as a GST-tag fusion protein in E. coli. After purification by GST-Agarose and subsequent GST-tag cleavage, the pure protein was yielded by gel filtration chromatography.
||Cofilin is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20 centigrade in glycerol or CryoProtect. Avoid repeated freeze / thaw cycles.
||At ambient temperature. Upon delivery store at -70 centigrade.