Recombinant Mouse Ch25h Protein, MYC/DDK-tagged


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Recombinant Mouse Ch25h Protein, MYC/DDK-tagged

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Cat.No. : Ch25h-883M
Product Overview : Purified recombinant protein of full-length mouse cholesterol 25-hydroxylase (Ch25h), with C-terminal MYC/DDK tag, was expressed in HEK293T cells.
Description : Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953). May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing (PubMed:9852097). In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation (PubMed:9852097).
Source : HEK293T
Species : Mouse
Molecular Mass : 34.7 kDa
Purity : >80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 µg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : Ch25h cholesterol 25-hydroxylase [ Mus musculus (house mouse) ]
Official Symbol : Ch25h
Synonyms : Ch25h; cholesterol 25-hydroxylase; m25OH; AI462618; cholesterol 25-hydroxylase; cholesterol 25-monooxygenase; EC
Gene ID : 12642
mRNA Refseq : NM_009890
Protein Refseq : NP_034020
UniProt ID : Q9Z0F5
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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