Recombinant Mouse Ch25h Protein, MYC/DDK-tagged

• Cat.No.: Ch25h-883M
• Product Overview: Purified recombinant protein of full-length mouse cholesterol 25-hydroxylase (Ch25h), with C-terminal MYC/DDK tag, was expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (PubMed:22999953). May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing (PubMed:9852097). In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation (PubMed:9852097).
• Molecular Mass: 34.7 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 µg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ch25h cholesterol 25-hydroxylase [ Mus musculus (house mouse) ]
• Official Symbol: Ch25h
• Synonyms: Ch25h; cholesterol 25-hydroxylase; m25OH; AI462618; cholesterol 25-hydroxylase; cholesterol 25-monooxygenase; EC 1.14.99.38
• Gene ID: 12642
• mRNA Refseq: NM_009890
• Protein Refseq: NP_034020
• UniProt ID: Q9Z0F5