Recombinant Mouse Chek1 Protein, His/GST-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Chek1-8670MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Chek1 (O35280-1) (Met1-Thr476), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.

Specification

• Source: Insect cells
• Species: Mouse
• Tag: His/GST
• Form: Lyophilized
• Molecular Mass: The recombinant mouse CHEK1/GST chimera consists of 713 amino acids and has a calculated molecular mass of 82.2 kDa. The recombinant protein migrates as an approximately 78 kDa band in SDS-PAGE under reducing conditions.
• Protein length: 713
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 3mM DTT, 10% gly
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Chek1 checkpoint kinase 1 [ Mus musculus ]
• Official Symbol: Chek1
• Gene ID: 12649
• mRNA Refseq: NM_007691
• Protein Refseq: NP_031717