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Recombinant Mouse chemokine (C-C motif) ligand 7 (CCL7)

Cat.No. : CCL7-46M
Product Overview : Recombinant Mouse Monocyte Chemotactic Protein-3 produced in E.Coli is a non-glycosylated, Polypeptide chain containing 74 amino acids and having a molecular mass of 8510 Dalton. The MCP-3 is purified by proprietary chromatographic techniques.
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Cat. No. : CCL7-46M
Description : Chemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). Due to CCL7 possessing two adjacent N-terminal cysteine residues in its mature protein, it is classified among the subfamily of chemokines known as CC chemokines. CCL7 specifically attracts monocytes, and regulates macrophage function. It is produced by certain tumor cell lines and by macrophages. This chemokine is located on chromosome 17 in humans, in a large cluster containing many other CC chemokines and is most closely related to CCL2 (previously called MCP1).
Source : Escherichia Coli.
Amino Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Ala-Gly-Pro.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Purity : Greater than 99.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation : The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives..
Solubility : It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-3 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Specific Activity : The specific activity as determined by the ability of Mouse MCP-3 to chemoattract Balb/C mouse spleen MNCs at 10-100 ng/ml.
Stability : Lyophilized MCP-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution CCL7 should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Publication :
Early gene activation initiates neuroinflammation prior to VSV neuroinvasion: Impact on antiviral responses and sleep (2017)

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