Recombinant Mouse Chemokine (C-X-C motif) Ligand 12

• Cat.No.: Cxcl12-371M
• Product Overview: Recombinant mouseCXCL12 is a non-glycosylated polypeptide chain containing 72 amino acids.

Specification

• Cat. No.: Cxcl12-371M
• Description: Cxcl12 is small cytokine belonging to the chemokinefamily that is officially designated Chemokine (C-X-C motif) ligand 12(CXCL12). Stromal cell-derived factors 1-alpha and 1-beta are small cytokinesthat belong to the intercrine family, members of which activate leukocytesand are often induced by proinflammatory stimuli such as lipopolysaccharide,TNF, or IL1. The intercrines are characterized by the presence of 4 conservedcysteines that form 2 disulfide bonds.
• Source: E. coli
• Species: Mouse
• Form: Lyophilized from a0.2 μm filtered concentrated (1.0mg/ml) solution in 20 mM PB, pH 7.4, 150 mMNaCl.
• Molecular Weight: 8.5 kDa
• Purity: >97% by SDS-PAGEand HPLC analyses.
• Endotoxin Level: < 0.1 ng/μg ofCXCL12.
• Biological Activity: Determined by itsability to chemoattract human peripheral blood monocytes using a concentrationrange of 50.0-100.0 ng/ml.
• Sequence: KPVSLSYRCPCRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRL KM
• Reconstitution: Centrifuge vialprior to opening. Add sterile distilled water or aqueous buffer containing0.1% BSA to a concentration of 0.1-1.0 mg/mL. Please note that the additionof any carrier protein into this product may produce unwanted endotoxin. Thisdepends upon the particular application employed. Further dilutions should bemade in appropriate buffered solutions.
• Storage: Stable at 2-8°C, butbest kept desiccated -20°C. Upon reconstitution, stable for up to 1 week at2-8°C. For longer term, store in working aliquots below -20°C. Avoid repeatedfreeze/thaw cycles.
• OfficialSymbol: Cxcl12

Gene Information

• Gene Name: Cxcl12 chemokine (C-X-C motif)ligand 12 [ Mus musculus ]
• Synonyms: Cxcl12; chemokine(C-X-C motif) ligand 12; Pbsf; Sdf1; Tlsf; Sdf1a; Sdf1b; Tlsfa; Tlsfb; Tpar1;Scyb12; AI174028; stromal cell-derived factor 1; OTTMUSP00000026112;OTTMUSP00000026113; OTTMUSP00000026114; C-X-C motif chemokine 12; pre-B cellgrowth-stimulating factor; pre-B-cell growth-stimulating factor; thymiclymphoma cell-stimulating factor; 12-O-tetradecanoylphorbol 13-acetaterepressed protein 1
• Gene ID: 20315
• mRNA Refseq: NM_013655
• Protein Refseq: NP_038683
• UniProt ID: P40224
• Chromosome Location: 6 F1; 6 53.0 cM
• Pathway: Axon guidance;Chemokine receptors bind chemokines; Chemokine signaling pathway; Class A/1(Rhodopsin-like receptors); Cytokine-cytokine receptor interaction; G alpha(i) signalling events; GPCR downstream signaling; GPCR ligand binding;Intestinal immune network for IgA production; Leukocyte transendothelialmigration; Peptide ligand-binding receptors; Rheumatoid arthritis; SignalTransduction; Signaling by GPCR
• Function: CXCR chemokinereceptor binding; chemokine activity; cytokine activity; growth factoractivity