Active Recombinant Mouse Cirbp protein, His-tagged

• Cat.No.: Cirbp-1779M
• Product Overview: Recombinant Mouse Cirbp protein(1-172 aa) was fused with a polyhistidine tag at the N-terminus.

Specification

• Species: Mouse
• Tag: His
• Protein Length: 1-172 aa
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Cold-inducible RNA-binding protein (CIRBP) plays a critical role in controlling the cellular response upon confronting a variety of cellular stresses, including short wavelength ultraviolet light, hypoxia, and hypothermia. Besides, Heterogeneous Nuclear Ribonucleoprotein A1 (HNRPA1) has been identified as an interactor of CIRBP, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse CIRBP and recombinant mouse HNRPA1. Briefly, CIRBP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to HNRPA1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CIRBP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CIRBP and HNRPA1 was shown, and this effect was in a dose dependent manner.
• Molecular Mass: 22.3kDa
• Purity: > 90%
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Cirbp cold inducible RNA binding protein [ Mus musculus ]
• Official Symbol: Cirbp
• Synonyms: CIRBP; cold inducible RNA binding protein; cold-inducible RNA-binding protein; A18 hnRNP; cold inducible RNA-binding protein; glycine-rich RNA-binding protein CIRP; Cirp; R74941
• Gene ID: 12696
• mRNA Refseq: NM_007705
• Protein Refseq: NP_031731