Recombinant Mouse Clec4e Protein, Myc/DDK-tagged

• Cat.No.: Clec4e-2187M
• Product Overview: Purified recombinant protein of mouse full-length C-type lectin domain family 4, member e (Clec4e), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: A calcium-dependent lectin that acts as a pattern recognition receptor of the innate immune system. Recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated molecular patterns (PAMPs) of bacteria and fungi. The PAMPs notably include mycobacterial trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid with potent adjuvant immunomodulatory functions. Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia and mediates macrophage activation. Through recognition of DAMPs released upon nonhomeostatic cell death, enables immune sensing of damaged self and promotes inflammatory cell infiltration into the damaged tissue.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 24.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Clec4e C-type lectin domain family 4, member e [ Mus musculus (house mouse) ]
• Official Symbol: Clec4e
• Synonyms: CLEC4E; C-type lectin domain family 4, member e; C-type lectin domain family 4 member E; C-type lectin superfamily member 9; macrophage-inducible C-type lectin; C-type lectin, superfamily member 9; C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 9; C86253; Mincle; Clecsf9
• Gene ID: 56619
• mRNA Refseq: NM_019948
• Protein Refseq: NP_064332
• UniProt ID: Q9R0Q8