| Cat. No. : |
Cntn6-462M |
| Description : |
Cntn6, also known asNB3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPIlinked neuronaladhesion proteins. Mature mouse Cntn6 consists of six immunoglobulinlike domains,a flexible linker region, four fibronectin type III domains, and a GPI anchor.Mouse Cntn6 shares 90% and 96% amino acid (aa) sequence identity with human andrat Cntn6, respectively. Alternate splicing generates an isoform with a 17 aadeletion in the first Iglike domain. |
| Source : |
Chinese Hamster Ovarycell line, CHO-derived |
| Form : |
Lyophilized from a 0.2μm filtered solution in PBS. |
| N-terminalSequence : |
Glu20 |
| Molecular Weight : |
109.2 kDa |
| Activity : |
Measuredby the ability of the immobilized protein to support the adhesion of Neuro2a mouseneuroblastoma cells (ATCC: CCL131). When 5 x 104 cells/well are addedto rmCntn6 coated plates (10 μg/mL, 100 μL/well), approximately 40-60% will adhereafter 30 minutes at 37 °C. Optimal dilutions should be determined by each laboratoryfor each application. |
| Endotoxin Level : |
<1.0 EUper 1 μg of the protein by the LAL method. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
Reconstituteat 100 μg/mL in sterile PBS |
| Storage : |
Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months,-20 to -70°C under sterile conditions after reconstitution. |
