Active Recombinant Mouse Ctsb protein, His-tagged

• Cat.No.: Ctsb-415M
• Product Overview: Recombinant Mouse Ctsb(Met 1-Phe 339) fused with His tag at C-terminal was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Predicted N Terminal: His 18
• Form: Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Z-LR-AMC. The specific activity is >2,000 pmoles/min/μg.
• Molecular Mass: The secreted recombinant mouse CTSB (pro form) consists of 333 amino acids and has a predicted molecular mass of 36.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CTSB is approximately 35-43 kDa due to glycosylation.
• Protein length: Met 1-Phe 339
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: Ctsb cathepsin B [ Mus musculus ]
• Official Symbol: Ctsb
• Synonyms: CTSB; cathepsin B; cathepsin B1; CB
• Gene ID: 13030
• mRNA Refseq: NM_007798
• Protein Refseq: NP_031824
• Chromosome Location: 14 D1; 14 33.24 cM
• Pathway: Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Lysosome, organism-specific biosystem; Lysosome, conserved biosystem; thyroid hormone biosynthesis, conserved biosystem
• Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; hydrolase activity; kininogen binding; peptidase activity; peptide binding; protein complex binding; protein self-association