Recombinant Mouse Ctsb protein, His-tagged

• Cat.No.: Ctsb-8176M
• Product Overview: Recombinant Mouse Ctsb aa. (Val129~Asp333 (Accession # P10605)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Val129~Asp333
• Description: This gene encodes a member of the peptidase C1 family and preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to generate the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. Homozygous knockout mice for this gene exhibit reduced pancreatic damage following induced pancreatitis and reduced hepatocyte apoptosis in a model of liver injury. Pseudogenes of this gene have been identified in the genome.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 23.7kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.8.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in ddH2O.

Gene Information

• Gene Name: Ctsb cathepsin B [ Mus musculus (house mouse) ]
• Official Symbol: Ctsb
• Synonyms: CB; cathepsin B; cathepsin B1
• Gene ID: 13030
• mRNA Refseq: NM_007798.3
• Protein Refseq: NP_031824.1
• UniProt ID: P10605