Active Recombinant Mouse Cyp2b10 Protein

• Cat.No.: Cyp2b10-121M
• Product Overview: Recombinant mouse Cyp2b10 protein and CYP-reductase without tag were coexpressed in E. coli.

Specification

• Description: Biased expression in duodenum adult (RPKM 61.9), lung adult (RPKM 58.0) and 2 other tissues
• Source: E. coli
• Species: Mouse
• Bio-activity: Cytochrome c Reductase Activity: 1989 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Bupropion hydroxylase
  Linear with time up to: 6 min(Determined at 15 μM substrate, using a P450 concentration of 6.25 pmol/ml)
  Linear with P450 up to: 6 pmol/ml(Determined at 15 μM substrate, using an incubation time of 5 min)
  Vmax: 30 pmol/min/pmol CYP
  Km: 18 μM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 7.1 nmol/ml
  Protein Concentration: 8.9 mg/ml
  Specific P450 Content: 798 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: Cyp2b10 cytochrome P450, family 2, subfamily b, polypeptide 10 [ Mus musculus (house mouse) ]
• Official Symbol: Cyp2b10
• Synonyms: Cyp2b10; cytochrome P450, family 2, subfamily b, polypeptide 10; p16; Cyp2b; Cyp2b20; cytochrome P450 2B10; CYPIIB10; CYPIIB20; P24; cytochrome P450 2B20; cytochrome P450 clone PF3/46; cytochrome P450, 2b10, phenobarbitol inducible, type b; cytochrome P450, 2b20; cytochrome P450, family 2, subfamily b, polypeptide 20; cytochrome P450-16-alpha; phenobarbitol inducible, type b; testosterone 16-alpha hydroxylase
• Gene ID: 13088
• mRNA Refseq: NM_009999
• Protein Refseq: NP_034129
• UniProt ID: Q9WUD0