Recombinant Mouse Dclk2 Protein, Myc/DDK-tagged

• Cat.No.: Dclk2-2474M
• Product Overview: Purified recombinant protein of mouse full-length doublecortin-like kinase 2 (Dclk2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmoduline-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. This gene and the DCX gene, another family member, share function in the establishment of hippocampal organization and their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 84.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dclk2 doublecortin-like kinase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Dclk2
• Synonyms: DCLK2; doublecortin-like kinase 2; serine/threonine-protein kinase DCLK2; caMK-like CREB regulatory kinase 2; doublecortin and CaM kinase-like 2; doublecortin-like and CAM kinase-like 2; CL2; CLICK2; Dcamkl2; AU044875; Click-II; 6330415M09Rik
• Gene ID: 70762
• mRNA Refseq: NM_001195496
• Protein Refseq: NP_001182425
• UniProt ID: Q6PGN3