Recombinant Mouse Dcun1d3 Protein, Myc/DDK-tagged

• Cat.No.: Dcun1d3-2485M
• Product Overview: Purified recombinant protein of mouse full-length DCN1, defective in cullin neddylation 1, domain containing 3 (S. cerevisiae) (Dcun1d3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Contributes to the neddylation of all cullins by transfering NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes and may play a role in the cell cycle progression by regulating the SCF ubiquitin E3 ligase complex, after UV damage. At the cell membrane, can promote and as well inhibit cullins neddylation.
• Molecular Mass: 34.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dcun1d3 DCN1, defective in cullin neddylation 1, domain containing 3 (S. cerevisiae) [ Mus musculus (house mouse) ]
• Official Symbol: Dcun1d3
• Synonyms: DCUN1D3; DCN1, defective in cullin neddylation 1, domain containing 3 (S. cerevisiae); DCN1-like protein 3; DCUN1 domain-containing protein 3; defective in cullin neddylation protein 1-like protein 3; BC030335; 1700020A13Rik; MGC40975
• Gene ID: 233805
• mRNA Refseq: NM_001163703
• Protein Refseq: NP_001157175
• UniProt ID: Q8K0V2