Recombinant Mouse Dgki Protein, Myc/DDK-tagged

• Cat.No.: Dgki-2539M
• Product Overview: Purified recombinant protein of mouse full-length diacylglycerol kinase, iota (Dgki), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Has probably no preference for any of the diacylglycerols in terms of the acyl chain composition, especially for the acyl chain at the sn-2 position. By controlling the diacylglycerol/DAG-mediated activation of RASGRP3, negatively regulates the Rap1 signaling pathway. May play a role in presynaptic diacylglycerol/DAG signaling and control neurotransmitter release during metabotropic glutamate receptor-dependent long-term depression.
• Molecular Mass: 118.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dgki diacylglycerol kinase, iota [ Mus musculus (house mouse) ]
• Official Symbol: Dgki
• Synonyms: DGKI; diacylglycerol kinase, iota; diacylglycerol kinase iota; C130010K08Rik
• Gene ID: 320127
• mRNA Refseq: NM_001081206
• Protein Refseq: NP_001074675
• UniProt ID: D3YWQ0