Recombinant Mouse Dhps Protein, Myc/DDK-tagged

• Cat.No.: Dhps-2547M
• Product Overview: Purified recombinant protein of mouse full-length deoxyhypusine synthase (cDNA clone MGC:49129 IMAGE:4240646), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a critical lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. This is the first step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.
• Molecular Mass: 20.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dhps deoxyhypusine synthase [ Mus musculus (house mouse) ]
• Official Symbol: Dhps
• Synonyms: DHPS; deoxyhypusine synthase; Dhs; MGC49129; MGC74384
• Gene ID: 330817
• mRNA Refseq: NM_001039514
• Protein Refseq: NP_001034603
• UniProt ID: Q3TXU5