Recombinant Mouse Dph1 Protein, MYC/DDK-tagged

• Cat.No.: Dph1-586M
• Product Overview: Purified recombinant protein of full-length mouse diphthamide biosynthesis 1 (Dph1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2. When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells. Acts also as a tumor suppressor in lung and breast cancers. Plays a role in embryonic growth, organogenesis and postnatal survival.
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 48.5 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dph1 diphthamide biosynthesis 1 [ Mus musculus (house mouse) ]
• Official Symbol: Dph1
• Synonyms: Dph1; diphthamide biosynthesis 1; Ovca1; Dph2l1; AW551873; 2310011M22Rik; 4930488F09Rik; 2-(3-amino-3-carboxypropyl)histidine synthase subunit 1; DPH1 homolog; DPH2-like 1; S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 1; diphthamide biosynthesis protein 1; diphthamide biosynthesis protein 2 homolog-like 1; diphtheria toxin resistance protein 1; diptheria toxin resistance protein required for diphthamide biosynthesis (Saccharomyces)-like 1; mmDph1; ovarian cancer-associated gene 1 protein homolog; EC 2.5.1.108
• Gene ID: 116905
• mRNA Refseq: NM_144491
• Protein Refseq: NP_652762
• UniProt ID: Q5NCQ5