Recombinant Mouse Dph5 Protein, Myc/DDK-tagged

• Cat.No.: Dph5-2636M
• Product Overview: Purified recombinant protein of mouse full-length diphthamide biosynthesis 5 (Dph5), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.
• Molecular Mass: 31.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dph5 diphthamide biosynthesis 5 [ Mus musculus (house mouse) ]
• Official Symbol: Dph5
• Synonyms: DPH5; DPH5 homolog (S. cerevisiae); diphthine synthase; diphthamide biosynthesis methyltransferase; C80186; AU045680; 2410012M04Rik
• Gene ID: 69740
• mRNA Refseq: NM_027193
• Protein Refseq: NP_081469
• UniProt ID: Q9CWQ0