Recombinant Mouse Dph6 Protein, Myc/DDK-tagged

• Cat.No.: Dph6-1780M
• Product Overview: Purified recombinant protein of mouse full-length ATP binding domain 4 (cDNA clone MGC:35721 IMAGE:5363234), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor 2 (EEF2) to diphthamide.
• Molecular Mass: 25 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dph6 diphthamine biosynthesis 6 [ Mus musculus (house mouse) ]
• Official Symbol: Dph6
• Synonyms: Dph6; diphthamine biosynthesis 6; Atpb; Atpbd4; 5730421E18Rik; diphthine--ammonia ligase; ATP binding domain 4; ATP-binding domain-containing protein 4; diphthamide synthase; diphthamide synthetase; protein DPH6 homolog; EC 6.3.1.14
• Gene ID: 66632
• mRNA Refseq: NM_025675
• Protein Refseq: NP_079951
• UniProt ID: Q9CQ28