Recombinant Mouse Dtd2 Protein, Myc/DDK-tagged

• Cat.No.: Dtd2-2668M
• Product Overview: Purified recombinant protein of mouse full-length D-tyrosyl-tRNA deacylase 2 (Dtd2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Probably acts by rejecting L-amino acids from its binding site rather than specific recognition of D-amino acids. Catalyzes the hydrolysis of D-tyrosyl-tRNA(Tyr), has no activity on correctly charged L-tyrosyl-tRNA(Tyr). By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality. In contrast to DTD1, deacylates L-Ala mischarged on tRNA(Thr)(G4.U69) by alanine-tRNA ligase AARS. Can deacylate L-Ala due to a relaxed specificity for substrate chirality caused by the trans conformation of the Gly-Pro motif in the active site. Also hydrolyzes correctly charged, achiral, glycyl-tRNA(Gly) in vitro, although in vivo EEF1A1/EF-Tu may protect cognate achiral glycyl-tRNA(Gly) from DTD2-mediated deacetylation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 18.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dtd2 D-tyrosyl-tRNA deacylase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Dtd2
• Synonyms: Dtd2; D-tyrosyl-tRNA deacylase 2; ATD; 4930578F06Rik; 6530401N04Rik; B830049N13Rik; D-aminoacyl-tRNA deacylase 2; L-alanyl-tRNA deacylase; animalia-specifc tRNA deacylase; animalia-specific tRNA deacylase; probable D-tyrosyl-tRNA(Tyr) deacylase 2; putative D-tyrosyl-tRNA deacylase 2; putative D-tyrosyl-tRNA(Tyr) deacylase 2; EC 3.1.1.96
• Gene ID: 328092
• mRNA Refseq: NM_029545
• Protein Refseq: NP_083821
• UniProt ID: Q8BHA3