"Edn1" Related Products

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Recombinant Mouse Edn1 protein, His-tagged

Cat.No.: Edn1-594M
Product Overview: Recombinant Mouse Edn1(Glu33~His202) fused with His tag at N-terminal was expressed in E. coli.
Description: This gene encodes a member of the endothelin family of peptides. The encoded preproprotein undergoes proteolytic processing to generate a peptide before secretion by the vascular endothelial cells. The mature peptide has various biological activities such as vasoconstriction, cell proliferation, stimulation of hormone release and modulation of central nervous activity. Mice lacking the encoded protein exhibit neonatal lethality accompanied with numerous craniofacial and cardiovascular defects due to disruption in cranial and cardiac neural crest cell patterning during early embryogenesis.
Source: E. coli
Species: Mouse
Tag: His
Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Molecular Mass: 21.0kDa
Protein length: Glu33~His202
Endotoxin: <1.0EU per 1µg (determined by the LAL method)
Purity: > 90%
Applications: SDS-PAGE; WB; ELISA; IP.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution: Reconstitute in PBS or others.
Gene Name: Edn1 endothelin 1 [ Mus musculus ]
Official Symbol: Edn1
Synonyms: EDN1; endothelin 1; endothelin-1; PPET1; preproendothelin; preproendothelin-1; ET-1; preproET;
Gene ID: 13614
mRNA Refseq: NM_010104
Protein Refseq: NP_034234
MIM:
UniProt ID: P22387
Chromosome Location: 13 A4; 13 20.82 cM
Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Melanogenesis, organism-specific biosystem; Melanogenesis, conserved biosystem; MicroRNAs in cardiomyocyte hypertrophy, organism-specific biosystem;
Function: cytokine activity; endothelin A receptor binding; endothelin A receptor binding; endothelin B receptor binding; hormone activity; protein binding; receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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