Recombinant Mouse Efnb3 protein(Met1-Ala227), His-tagged

• Cat.No.: Efnb3-985M
• Product Overview: Recombinant Mouse Efnb3 (NP_031937.1) (Met1-Ala227) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-227 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EFNB3-His at 10μg/mL (100μL/well) can bind biotinylated mouse EPHB3-His, the EC50 of biotinylated mouse EPHB3-His 0.02-0.4 μg/mL.
• Molecular Mass: The recombinant mouse Efnb3 consists 211 amino acids and predicts a molecular mass of 23.5 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Efnb3 ephrin B3 [ Mus musculus ]
• Official Symbol: Efnb3
• Synonyms: EFNB3; ephrin B3; ephrin-B3; Epl8; EFL-6; ELF-3; Elk-L3; LERK-8; NLERK-2
• Gene ID: 13643
• mRNA Refseq: NM_007911
• Protein Refseq: NP_031937