Recombinant Mouse Eif5 Protein, Myc/DDK-tagged

• Cat.No.: Eif5-2791M
• Product Overview: Purified recombinant protein of mouse full-length eukaryotic translation initiation factor 5 (Eif5), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
• Molecular Mass: 49 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Eif5 eukaryotic translation initiation factor 5 [ Mus musculus (house mouse) ]
• Official Symbol: Eif5
• Synonyms: EIF5; eukaryotic translation initiation factor 5; eIF-5; D12Ertd549e; 2810011H21Rik; MGC36374; MGC36509
• Gene ID: 217869
• mRNA Refseq: NM_173363
• Protein Refseq: NP_775539
• UniProt ID: P59325