Recombinant Mouse Endov Protein, Myc/DDK-tagged

• Cat.No.: Endov-2822M
• Product Overview: Purified recombinant protein of mouse full-length endonuclease V (Endov), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine. Active against both single-stranded and double-stranded RNAs. Has strong preference for single-stranded RNAs (ssRNAs) toward double-stranded RNAs (dsRNAs). Cleaves mRNAs and tRNAs containing inosine. Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3'. Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing. Binds branched DNA structures.
• Molecular Mass: 28 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Endov endonuclease V [ Mus musculus (house mouse) ]
• Official Symbol: Endov
• Synonyms: Endov; endonuclease V; A730011L01Rik; endonuclease V; novel endonuclease V protein; putative endonuclease FLJ39025 homolog; EC 3.1.-.-
• Gene ID: 338371
• mRNA Refseq: NM_177394
• Protein Refseq: NP_796368
• UniProt ID: Q8C9A2