Recombinant Mouse Epha3 Protein, His-GST-tagged, Alexa Fluor 555 conjugated

• Cat.No.: EPHA3-180MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Epha3 (EDK98238.1) (Gly569-Val984), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 653
• Form: Lyophilized
• Molecular Mass: The recombinant mouse EPHA3/GST chimera consists of 653 amino acids and has a calculated molecular mass of 74.3 kDa. The recombinant protein migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.
• N-terminal Sequence Analysis: Met
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Alexa Fluor 555

Gene Information

• Gene Name: Epha3 Eph receptor A3 [ Mus musculus ]
• Official Symbol: Epha3
• Gene ID: 13837
• mRNA Refseq: NM_010140
• Protein Refseq: NP_034270