Recombinant Mouse Erbb2 Protein, MYC/DDK-tagged

• Cat.No.: Erbb2-969M
• Product Overview: Purified recombinant protein of full-length mouse v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian),, with C-terminal MYC/DDK tag, was expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Broad expression in colon adult (RPKM 50.3), duodenum adult (RPKM 35.2) and 17 other tissues
• Molecular Mass: 172 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 µg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Erbb2 erb-b2 receptor tyrosine kinase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Erbb2
• Synonyms: Erbb2; erb-b2 receptor tyrosine kinase 2; Neu; HER2; HER-2; c-neu; Erbb-2; c-erbB2; l11Jus8; mKIAA3023; receptor tyrosine-protein kinase erbB-2; Neu oncogene; avian erythroblastosis oncogene B 2; c-erbB-2; p185erbB2; proto-oncogene NEU; proto-oncogene c-ErbB-2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; EC 2.7.10.1
• Gene ID: 13866
• mRNA Refseq: NM_001003817
• Protein Refseq: NP_001003817
• UniProt ID: P70424

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.