Active Recombinant Mouse F7 protein(Met1-Leu446), His-tagged

• Cat.No.: F7-2206M
• Product Overview: Recombinant Mouse FⅦ (NP_034302.2) (Met 1-Leu 446) was expressed in CHO, fused with the a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: CHO
• Tag: His
• Protein Length: Met1-Leu446
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse F7-his at 10 μg/ml (100 μl/well) can bind biotinylated mouse F3-his. The EC50 of biotinylated mouse F3-his is 0.1-0.3 μg/ml.
• Molecular Mass: The mature form of mouse FⅦ consists of 416 amino acids after removal of the signal peptide and the propeptid, and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FⅦ is approximately 56-63 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: F7 coagulation factor VII [ Mus musculus ]
• Official Symbol: F7
• Synonyms: F7; coagulation factor VII; serum prothrombin conversion accelerator; Cf7; FVII; mfVII; AI132620
• Gene ID: 14068
• mRNA Refseq: NM_010172
• Protein Refseq: NP_034302