Recombinant Mouse Fas protein, His-tagged

• Cat.No.: Fas-617M
• Product Overview: Recombinant Mouse Fas (Q8C350) aa. (Gln22~Arg169) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gln22~Arg169
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 7.8.
• Applications: Positive Control; Immunogen; SDS-PAGE; WB
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Fas Fas (TNF receptor superfamily member 6) [ Mus musculus (house mouse) ]
• Official Symbol: Fas
• Synonyms: Fas; Fas (TNF receptor superfamily member 6); lpr; APO1; APT1; CD95; TNFR6; Tnfrsf6; AI196731; tumor necrosis factor receptor superfamily member 6; FASLG receptor; Fas (TNF receptor superfamily member); Fas antigen; apo-1 antigen; apoptosis-mediating surface antigen FAS; lymphoproliferation
• Gene ID: 14102
• mRNA Refseq: NM_001146708.1
• Protein Refseq: NP_001140180.1
• UniProt ID: Q8C350

Reviews

09/07/2022

When experimenting with FAS, the results are very reproducible.

07/29/2022

The stability of FAS products allows them to cope with different experimental conditions and modes of operation.

09/20/2021

FAS has high specific activity and can exert strong biological effect at low concentration.

Q&As

Does FAS protein play a role in cell signaling?

FAS protein plays an important role in cell signaling, it can activate downstream signaling pathways, such as death signaling pathway and survival signaling pathway, by binding to its ligand and transmitting signals.

Why apply knowledge of FAS proteins to clinical practice?

It can provide a reference for clinical practice and guide the diagnosis, treatment and prevention of diseases by detecting the expression level of FAS protein and understanding its mechanism of action.

How can diseases associated with FAS protein be prevented and treated?

FAS protein-related diseases can be prevented and treated by regulating the expression level of FAS protein and inhibiting its signaling pathway.

How can gene knockout or knockdown techniques be used to study the function of FAS proteins?

Gene knockout or knockdown techniques can be used to study the function and mechanism of action of FAS proteins. By knocking out or knocking down the FAS gene, changes in processes such as apoptosis and immune response can be observed to further understand its function and mechanism of action.

What is the role of FAS protein in apoptosis?

FAS protein can bind to its ligand, activate the death signaling pathway, and induce apoptosis.

How can FAS protein be used to predict disease prognosis?

The expression level and activity of FAS protein can be measured to predict the prognosis and development trend of certain diseases.