Recombinant Mouse Fen1 Protein, Myc/DDK-tagged

• Cat.No.: Fen1-2986M
• Product Overview: Purified recombinant protein of mouse full-length flap structure specific endonuclease 1 (Fen1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
• Molecular Mass: 42.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Fen1 flap structure specific endonuclease 1 [ Mus musculus (house mouse) ]
• Official Symbol: Fen1
• Synonyms: FEN1; flap structure specific endonuclease 1; flap endonuclease 1; FEN-1; flap structure-specific endonuclease 1; AW538437
• Gene ID: 14156
• mRNA Refseq: NM_007999
• Protein Refseq: NP_032025
• UniProt ID: Q91Z50