Active Recombinant Mouse Fgf1 protein

Name: Active Recombinant Mouse Fgf1 protein
SKU: Fgf1-558M

Specification
Gene Information
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Species :	Mouse
Source :	E.coli
Tag :	Non
Protein Length :	16-155 aa
Form :	Lyophilized from a 0.2 μm filtered concentrated solution in 1×PBS, pH7.0, 5%Trehalose, 0.02%Tween-80, 0.5mM DTT, 0.5mM EDTA
Bio-activity :	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 10^6 IU/mg in the presence of 10 μg/ml of heparin.
Molecular Mass :	Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 140 amino acid residues.
AASequence :	FNLPLGNYKK PKLLYCSNGG HFLRILPDGT VDGTRDRSDQ HIQLQLSAES AGEVYIKGTE TGQYLAMDTE GLLYGSQTPN EECLFLERLE ENHYNTYTSK KHAEKNWFVG LKKNGSCKRG PRTHYGQKAI LFLPLPVSSD
Endotoxin :	Less than 1 EU/μg of rMuaFGF as determined by LAL method.
Purity :	> 96 % by SDS-PAGE and HPLC analyses.
Storage :	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution :	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Name	Fgf1 fibroblast growth factor 1 [ Mus musculus ]
Official Symbol	Fgf1
Synonyms	FGF1; fibroblast growth factor 1; aFGF; HBGF-1; acidic fibroblast growth factor; heparin-binding growth factor 1; fibroblast growth factor 1 (acidic); Fam; Fgfa; Dffrx; Fgf-1;
Gene ID	14164
mRNA Refseq	NM_010197
Protein Refseq	NP_034327