Active Recombinant Mouse Folh1 protein, His-tagged, Low Endotoxin

• Cat.No.: Folh1-4533M
• Product Overview: Recombinant Mouse Folh1 protein(O35409)(45-752 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 45-752 aa
• Form: Lyophilized from 0.22 μm filtered solution in 50mM MES, 150mM NaCl, 8% trehalose (pH 6.0).
• Bio-activity: Measured by its ability to hydrolyze the substrate N-acetyl-L-Asp-L-Glu into N-acetyl-L-Asp and L-Glu. The L-Glu product is measured by fluorescence after its derivatization by ortho-phthaldialdehyde. The specific activity is >500 pmol/min/µg.
• Molecular Mass: The protein has a predicted MW of 80.6 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 90% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Folh1 folate hydrolase [ Mus musculus ]
• Official Symbol: Folh1
• Synonyms: FOLH1; folate hydrolase; glutamate carboxypeptidase 2; FGCP; mGCP; GCPII; NAALADase I; folate hydrolase 1; glutamate carboxypeptidase II; membrane glutamate carboxypeptidase; folylpoly-gamma-glutamate carboxypeptidase; prostate-specific membrane antigen homolog; pteroylpoly-gamma-glutamate carboxypeptidase; N-acetylated-alpha-linked acidic dipeptidase I; GCP2; mopsm; MGC141397
• Gene ID: 53320
• mRNA Refseq: NM_001159706
• Protein Refseq: NP_001153178