Recombinant Mouse FST protein(Met1-Asn317), hFc-tagged

• Cat.No.: FST-2333M
• Product Overview: Recombinant Mouse FST (P47931) (Met 1-Asn 317) was expressed in CHO with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Mouse
• Source: CHO
• Tag: Fc
• Protein Length: Met1-Asn317
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 40-200 ng/mL in the presence of 10 ng/mL rhActivin A.
• Molecular Mass: The recombinant mouse FST comprises 528 amino acids and has a predicted molecular mass of 58.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 66 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fst follistatin [ Mus musculus ]
• Official Symbol: FST
• Synonyms: FST; follistatin; FS; activin-binding protein; AL033346
• Gene ID: 14313
• mRNA Refseq: NM_008046
• Protein Refseq: NP_032072